Passive and facilitated transport in nuclear pore complexes is largely uncoupled.

نویسندگان

  • Bracha Naim
  • Vlad Brumfeld
  • Ruti Kapon
  • Vladimir Kiss
  • Reinat Nevo
  • Ziv Reich
چکیده

Nuclear pore complexes provide the sole gateway for the exchange of material between nucleus and cytoplasm of interphase eukaryotic cells. They support two modes of transport: passive diffusion of ions, metabolites, and intermediate-sized macromolecules and facilitated, receptor-mediated translocation of proteins, RNA, and ribonucleoprotein complexes. It is generally assumed that both modes of transport occur through a single diffusion channel located within the central pore of the nuclear pore complex. To test this hypothesis, we studied the mutual effects between transporting molecules utilizing either the same or different modes of translocation. We find that the two modes of transport do not interfere with each other, but molecules utilizing a particular mode of transport do hinder motion of others utilizing the same pathway. We therefore conclude that the two modes of transport are largely segregated.

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Passive and Facilitated Transport in Nuclear Pore Complexes

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Naim et al Text_revision_211106

Nuclear pore complexes (NPCs) provide the sole gateway for the exchange of material between nucleus and cytoplasm of interphase eukaryotic cells. They support two modes of transport: passive diffusion of ions, metabolites and intermediate-sized macromolecules, and facilitated, receptor-mediated translocation of proteins, RNA, and RNPs complexes. It is generally assumed that both modes of transp...

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 282 6  شماره 

صفحات  -

تاریخ انتشار 2007